The rpob gene as a tool for clinical microbiologists

This same kind of mechanism was described in the case of C. It should be noted that there appears to be a strong association between C. On the contrary, C. If the association between clostridia and NEC is increasingly being reported, the pathogeny of this disease remains elusive in spite of the usual suggestion of toxin production, as in the case of C. Herein, genes encoding the secretion of bacterial toxins were predicted, especially haemolysin and C.

These latter are a leading cause of CDI, where the pathogenic mechanism is the consequence of TcdA enterotoxin and TcdB cytotoxin production inducing colonic tissue damage [ 25 , 26 ]. The idea of toxin-mediated disease rather than invasion-mediated disease is supported by the work of Heida et al. Furthermore, haemolysin sequences with a highly conserved domain found in a toxin of Brachyspira hyodysenteriae showed a cytolytic effect in several cell lines [ 13 ].

Cassir et al. In conclusion, this study highlights the association between C. Further genomic analysis is required on a larger number of sequenced genomes. None of the authors have any conflicts of interest to report.

National Center for Biotechnology Information , U. New Microbes New Infect. Published online Oct Hosny , E. Baptiste , A. Levasseur , and B. Author information Article notes Copyright and License information Disclaimer.

La Scola: rf. This article has been cited by other articles in PMC. Associated Data Supplementary Materials Multimedia component 1. Abstract Clostridia—especially Clostridium butyricum —are among the taxa most frequently identified from stool samples of preterm neonates with necrotizing enterocolitis NEC. Keywords: Clonal lineage, Clostridium neonatale , Core-genome phylogeny, Necrotizing enterocolitis, rpoB-based specific quantitative real-time polymerase chain reaction.

Introduction Necrotizing enterocolitis NEC is a major cause of morbidity and mortality in neonatal intensive care units NICUs , especially for very-low-birth-weight infants [ 1 ]. Table 1 Factors associated with the occurrence of necrotizing enterocolitis NEC compared with control preterm neonates.

Open in a separate window. SD, standard deviation; NA, not applicable. Genome sequencing, assembly and annotation of Clostridium neonatale isolates Five C. Table 2 Characteristics of Clostridium neonatale draft genomes. Draft genome-based and core-genome phylogeny For draft genome-based phylogeny, only mapped genomes were included.

Results Evaluation of patient features and Clostridium neonatale- specific rpoB -based qPCR system There was no statistically significant difference between NEC and control groups regarding gestational age, days of life, sex ratio, birth weight and feeding strategies.

Genomic analysis of Clostridium neonatale Final draft genomes of C. Discussion Dysbiosis remains a major risk factor inducing the establishment of NEC by promoting the translocation of pathogenic bacteria. Conflict of interest None of the authors have any conflicts of interest to report. Appendix A. Supplementary data The following is the Supplementary data to this article: Multimedia component 1: Click here to view.

References 1. Hosny M. Updating on gut microbiota and its relationship with the occurrence of necrotizing enterocolitis. Hum Microbiome J. Cassir N. Gut microbiota and the pathogenesis of necrotizing enterocolitis in preterm neonates. Future Microbiol. Clostridium butyricum strains and dysbiosis linked to necrotizing enterocolitis in preterm neonates. Clin Infect Dis. Ward D. Metagenomic sequencing with strain-level resolution implicates uropathogenic E.

Cell Rep. Boccia D. Nosocomial necrotising enterocolitis outbreaks:epidemiology and control measures. Eur J Pediatr. Smith B. Community analysis of bacteria colonizing intestinal tissue of neonates with necrotizing enterocolitis.

BMC Microbiol. Multidisciplinary evaluation of Clostridium butyricum clonality isolated from preterm neonates with necrotizing enterocolitis in South France between and Sci Rep. Alfa M.

An outbreak of necrotizing enterocolitis associated with a novel Clostridium species in a neonatal intensive care unit. Roze J. Nutritional strategies and gut microbiota composition as risk factors for necrotizing enterocolitis in very-preterm infants.

Am J Clin Nutr. Benamar S. Genome sequence of a Clostridium neonatale strain isolated in a Canadian neonatal intensive care unit. Genome Announc. Bernard K. Clostridium neonatale sp. Lawson and Rainey Int J Syst Evol Microbiol. Genomics, Proteomics Bioinforma. Description of Clostridium phoceensis sp.

New Microbe. New Infect. Either your web browser doesn't support Javascript or it is currently turned off. In the latter case, please turn on Javascript support in your web browser and reload this page. Read article at publisher's site DOI : Microbiol Spectr , 9 3 :e, 24 Nov Diagnostics Basel , 11 9 , 15 Sep Genome Med , 13 1 , 13 Sep BMC Microbiol , 21 1 , 28 Jul Microorganisms , 9 4 , 27 Mar This data has been provided by curated databases and other sources that have cited the article.

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